- 作者: Guozhen Chen; Jing Zhu; Tiewei Lv; Gang Wu; Huichao Sun; Xupei Huang; Jie Tian
- 作者服務機構: Department of Cardiology, the Children's Hospital of Chongqing Medical University, China
- 中文摘要: --
- 英文摘要:
Histone acetyltransferases (HATs), p300 and cAMP response element binding protein
(CREB)-binding protein (CBP) are two structurally related transcriptional co-activators that
activate expression of many eukaryotic genes involved in cellular growth and signaling,
muscle differentiation and embryogenesis. However, whether these proteins play important
and different roles in mouse cardiogenesis is not clear. Here, we investigate the protein
distributions and mRNA expression of the two HATs in embryonic and adult mouse heart
during normal heart development by using immunohistochemical and RT–PCR techniques.
The data from immunohistochemical experiments revealed that p300 was extensively present
in nearly every region of the hearts from embryonic stages to the adulthood. However, no
CBP expression was detected in embryonic hearts at day E7.5. CBP expression appeared at
the later stages, and the distribution of CBP was less than that of p300. In the developmental
hearts after E10.5, both for p300 and CBP, the mRNA expression levels reached a peak on
day E10.5, and then were gradually decreased afterwards. These results reveal that both p300
and CBP are related to embryonic heart development. The dynamic expression patterns of
these two enzymes during mouse heart development indicate that they may play an important
role on heart development. However, there is a difference in spatiotemporal expression
patterns between these two enzymes during heart development. The expression of p300 is
earlier and more predominate, suggesting that p300 may play a more important role in
embryonic heart development especially during cardiac precursor cell induction and
interventricular septum formation. - 中文關鍵字: --
- 英文關鍵字: --