- 作者: Mohammad Mahdi Forghanifard, Meysam Moghbeli, Reza Raeisossadati, Alireza Tavassoli, Afsaneh Javdani Mallak, Samaneh Boroumand-Noughabi and Mohammad Reza Abbaszadegan
- 作者服務機構: Department of Biology, Damghan Branch, Islamic Azad University, Cheshmeh- Ali Boulevard, Sa’dei Square, P.O.Box: 3671639998, Damghan, Iran
- 中文摘要: --
- 英文摘要:
Background: Human cancer cells resemble stem cells in expression signatures leading them to share some
features, most notably, self-renewal. A complex network of transcription factors and
signaling molecules are required for continuance of this trait. SALL4 is a zinc finger
transcriptional activator crucial for maintenance of self-renewal in stem cells; however, its
expression level has not yet been elucidated in colorectal tumor cells. To determine this level
and probable clinicopathological consequences, its expression was analyzed.
Methods: SALL4 expression in fresh tumoral and distant tumor-free tissues from 46 colorectal samples
was compared by real-time polymerase chain reaction (PCR).
Results: Greater than a two-fold increase in SALL4 expression was detected in 87% of tumors vs.
normal related tissues. SALL4 expression was significantly correlated with tumor cell
metastasis to lymph nodes, especially in moderately-differentiated tumor samples (P < 0.05).
Furthermore, higher levels of SALL4 mRNA expression were significantly associated with
younger than older patients with tumor cells in stages I and II (P < 0.05).
Conclusions: These results indicate a relationship between SALL4 expression and tumor cell metastasis to
lymph nodes and consequent advancement of tumors to advanced stages III and IV. Along
with the promising evidence of its role in self-renewal in various cancers, SALL4 may have a
role in progression, development and maintenance of colorectal cancers. - 中文關鍵字: --
- 英文關鍵字: Colorectal cancer, SALL4, Expressional analysis, Real-time PCR, Self-renewal