- 作者: Wen-Kwei Chen; Nan-Chi A. Chang; Yen-Hwa Chang; Kuo-Long Chang Shinn-Chih Wu; Tzong-Shang Yang; Sheue-Mei Wu; Alice Chien Chang
- 作者服務機構: Institues of Microbilogy and Immunology and Neuroscience, Center for Neurosciens, National Yang-Ming University, and Department of Surgery, Veterans General Hospital ,Taiei, and Division of Biotechnology, Animal Technology Institute of Taiwan, Miaoli, Taiwan, ROC
- 中文摘要: --
- 英文摘要: The 5' flanking sequence (3,227 base pairs, by)of themouse Adra2c subtype gene was determined and char-acterized. The transcription start site was mapped tonucleotide'A' of two initiator motifs in tandem array, i.e.1,159 and 1,153 bp upstream from the initiation codon ofthe open reading frame(ORF)of Adra2c, respectively.One structural feature salient to the 5' regulatory regionof Adra2c is present in the sequence 1 kb immediatelyupstream from the receptor ORF, which is highly en-riched in GC content (76%)and CpG island counts(i.e.CpG/GpC, 146:177),and thus rich in Sp1一binding motifs.At the 3' flanking region,the polyadenylation signal wasmapped to 481 by downstream from the terminationcodon.The transcript defined by sequence data therebyis consistent with a size of 3 kb(brain form)determinedby Northern blot analysis. The transgene, Adra2c-NN-IacZ, which links the promoter region of Adra2c to theIacZreporter gene, was constructed in order to evaluatethe functional capacity of the promoter and the putativemotifs residing within the defined regulatory region(1.9 kb upstream from the ORF) in directing the reportergene expression in vitro in transiently transfected cellsand in vivo in transgenic (Tg)mice.Permissive cell typesto Adra2c-NN include those derived from neural and kid-ney lineages. Significant Adra2c-NN-driven reporter ex-pression in Tg mice established suggests that α2C adre-noceptor expression is permissive under Adra2c-NN incentral (cerebral cortex, hippocampus, subthalamus, hy-pothalamus, superior colliculus, cerebellum,and brainstem)and peripheral(pancreatic (β-islets) tissues.
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