- 作者: Sun-Long Cheng; Rosa Huang Liu; Jin-Nan Sheu; Shui-Tein Chen; Supachok Sinchaikul; Gregory Jiazer Tsay
- 作者服務機構: 1 Institute of Medicine, Chung Shan Medical University, Taichung, 40242, Taiwan; ; 2 School of Nutrition, Chung Shan Medical University, Taichung, 40242, Taiwan; ; 3 Institute of Biological Chemistry and Genomics Research Center, Academia S璯ica, Taipei, 11529, Taiwan; ; 4 Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, 10617, Taiwan; ; 5 Institute of Immunology, Chung Shan Medical University, 110 Sec. 1, Chien-Kuo N. Road, Taichung, 40242, Taiwan
- 中文摘要: --
- 英文摘要: Although arbutin is a natural product and widely used as an ingredient in skin care products, its effect on the gene expression level of human skin with malignant melanoma cells is rarely reported. We aim to investigate the genotoxic effect of arbutin on the differential gene expression profiling in A375 human malignant melanoma cells through its effect on tumorigenesis and related side-effect. The DNA microarray analysis provided the differential gene expression pattern of arbutin-treated A375 cells with the significant changes of 324 differentially expressed genes, containing 88 up-regulated genes and 236 down-regulated genes. The gene ontology of differentially expressed genes was classified as belonging to cellular component, molecular function and biological process. In addition, four down-regulated genes of AKT1, CLECSF7, FGFR3, and LRP6 served as candidate genes and correlated to suppress the biological processes in the cell cycle of cancer progression and in the downstream signaling pathways of malignancy of melanocytic tumorigenesis.
- 中文關鍵字: --
- 英文關鍵字: arbutin, A375 cells, toxicogenomics, DNA microarray, gene expression