• 作者： 余家利; 蔡長祐; 謝松洲; 蔡瀛陽; 蔡世滋; 孫光蕙; 余幸司; 韓韶華
• 作者服務機構： 國立陽明大學醫學院過敏免疫風濕科; 台北榮民總醫院家庭醫學科; 國立陽明大學醫事技術學系; 高雄醫學院皮膚科
• 中文摘要： 　　健常人的周邊多形核白血球在體外培養時，可以自發地產生補體第三成分。這個補體產生能力可以被腫瘤壞死因子－α(20 ng/ml)或細菌性脂多醣體(100 ng/ml)所增強，但不為第一介白質－β及第八介白質所影響。這種補體產生能力是溫度依存性，且可為蛋白質抑制劑所壓抑。經腫瘤壞死因子－α或細菌性脂多醣體刺激六小時之後，以PT-PCR的方法可以測定出白血球細胞內有C3mRNA的表現。更者，我們比較了十五位活動性類風濕關節炎，十五位非活動性類風濕關節炎，以及十五位健常人周邊白血球自發性以及經腫瘤壞死因子－α刺激產生補體第三成分之能力。我們無法證實三組病人之間有意義的差異，顯示多形核白血球在活動性類風濕關節病人血中C3高補體血症中不具角色。
• 英文摘要： Normal human polymorphonuclear neutrophils (PMN) can spontaneously produce the third com-ponent of complement (C3) in in vitro culture as detected by ELISA. This C3-producing capacity of PMNcan be augmented by TNF- (20 ng/ml) and bacterial lipopolysaccharide (100 ng/ml), but not by IL-1or IL-8. The C3 production by PMN was found to be temperature dependent and was suppressed by theaddition of protein inhibitor. The C3 mRNA in PMN could be detected by reverse transcription assistedpolymerase chain reaction (RT-PCR) after TNF- or LPS stimulation for 6 hours. To further understandC3 production by peripheral blood PMN in rheumatoid arthritis (RA), spontaneous and TNF- stimulatedproduction of C3 by peripheral PMN were compared in 15 cases of active RA, 15 inactive RA and 15normal individuals. We failed to find any significant difference among the three groups. We concludethat PMN plays a negligible role in C3 hypercomplementemia in patients with active RA.
• 中文關鍵字： complement C3; polymorphonuclear neutrophil; tumor necrosis factor-α; bacterial lipopolysac-charide; rheumatoid arthritis.
• 英文關鍵字： --