- 作者: 蕭志遠、孫嬿、駱文、林頌然、紀秀華、詹國偵、董成淵
- 作者服務機構: Department of Physics, National Taiwan University, Taipei 106, Taiwan, R.O.C.
- 中文摘要: Multiphoton generated autofluorescence and second harmonic signals are useful luminescent signatures for probing the structural and spectral properties of collagen fibers. However, optimization of image formation deep within a three-dimensional collagen sample may be hindered by index-mismatch induced spherical aberration. In this work, we examined this issue by comparing the axial multiphoton luminescence profiles of bovine tendon under water, glycerin, and oil immersion conditions. Our results show that, although in general, oil immersion imaging resulted in the slowest decay of measured axial luminescence profile, other immersion conditions can perform better at specific locations within the tendon, indicating the existence of local variations of refractive indices within the collagen-filled tendon. Our results suggest that optimizing multiphoton imaging of collagen requires acquiring and comparing imaging results using different immersion fluids and that the results may be used to infer the spatial distribution of refractive indices in biological specimens.
- 英文摘要: --
- 中文關鍵字: Multiphoton microscopy; Second harmonic; Fluorescence; Collagen; Spherical aberration.
- 英文關鍵字: --