- 作者: Ching-Hua Hsieh, Johnson Chia-Shen Yang, Jonathan Chris Jeng, Yi-Chun Chen, Tsu-Hsiang Lu, Siou-Ling Tzeng, Yi-Chan Wu, Chia-Jung Wu and Cheng-Shyuan Rau
- 作者服務機構: Department of Plastic and Reconstructive Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung City, Taiwan, R.O.C
- 中文摘要: --
- 英文摘要:
Background: Previously, we had identified a specific whole blood–derived microRNAs (miRNAs)
signature in mice following in vivo injection of lipopolysaccharide (LPS) originated from
Gram-negative bacteria. This study was designed to profile the circulating miRNAs
expression in mice exposed to lipoteichoic acid (LTA) which is a major component of the
wall of Gram-positive bacteria.
Results: C57BL/6 mice received intraperitoneal injections of 100 μg of LTA originated from Bacillus
subtilis, Streptococcus faecalis, and Staphylococcus aureus were killed 6 h and the whole
blood samples were obtained for miRNA expression analysis using a miRNA array (Phalanx
miRNA OneArray® 1.0). Up-regulated expression of miRNA targets in the whole blood,
serum and white blood cells (WBCs) of C57BL/6 and Tlr2−/− mice upon LTA treatment in
10, 100, or 1000 ug concentrations was quantified at indicated time (2, 6, 24, and 72 h) using
real-time RT-PCR and compared with that in the serum of C57BL/6 mice injected with 100
ug of LPS. A significant increase of 4 miRNAs (miR-451, miR-668, miR-1902, and miR-
1904) was observed in the whole blood and the serum in a dose- and time-dependent fashion
following LTA injection. Induction of miRNA occurred in the serum after 2 h and persisted
for at least 6 h. No increased expression of these 4 miRNAs was found in the WBCs. Higher
but not significant expression level of these 4 miRNAs were observed following LTA
treatment in the serum of Tlr2−/−against that of C57BL6 mice. In contrast, LPS exposure
induced moderate expression of miR-451 but not of the other 3 miRNA targets.
Conclusions: We identified a specific circulating miRNA signature in mice exposed to LTA. That
expression profile is different from those of mice exposed to LPS. Those circulating miRNAs
induced by LTA or LPS treatment may serve as promising biomarkers for the differentiation
between exposures to Gram-positive or Gram-negative bacteria. - 中文關鍵字: --
- 英文關鍵字: MicroRNAs, Lipoteichoic acid, Lipopolysaccharide, Toll-like receptor, Gram-positive bacteria, Gram-negative bacteria, Microarray