• 作者： 章麗雲; 蔡珊珊
• 作者服務機構： 國立台灣大學植物學系
• 中文摘要： 米酒廠蒸餾廢液可被 Aspergillus niger F-0213 用做檸檬酸醱酵之培養基。由於廢液含糖量低，必須添加適當之碳源。本實驗選擇蔗糖做為碳源。磷酸鹽之添加，對菌絲生長量只有少許影響，但檸檬酸之產量降低很多。添加4%甲醇或0.05%黃血鹽，很明顯地提高了檸檬酸產量。微量元素鋅鹽之添加，不但不會提高檸檬酸產量，且與鎂鹽同時存在時，反而有抑制作用。添加氮源，可增加菌絲生長量及糖消耗量，而唯有加入低濃度（0.2%）之硝酸鈉或尿素，能提高檸檬酸產量。廢液之 pH 值若調高至 pH4，則檸檬酸產量達消耗糖之 73.59%。除不添加氮源外，在6%糖濃度下，總合上述有利條件，且接種砲子懸浮液 2.4ml/100m1 之培養液，於28°C表面培養，會使培養液之 BOD 降低 81.55%，產酸達消耗糖之80.29%；對原料糖收率為78.15%。
• 英文摘要： The distilled slop of rice liquor wasused as a fermentation medium for theproduction of citric acid by Aspergillusniger F-0213. Since the sugar contentin the distilled slop was low, the addi-tion of sucrose as a carbon source forthe fermentation is needed. The addi-tion of phosphate appearedto have little effect on mycelial growth,but markedly reduced the formation ofcitric acid. Methanol at a concentrationof 4% or potassium ferrocyanide at0.05% can increase the amount of citricacid markedly. The element magnesiumcould stimulate the production of citr-ate, however, the addition of zincelement not only had no effect oncitrate formation, but cancelled out thestimulation effect caused by magnesiumwhen elements zinc and magnesiumadded together. The addition of nitro-gen cnmpound increased mycelial grow-th and the consumption of sugar, butonly small amount (0.2%) of orurea added could increase the amountof citrate produced. The pH value offermentation medium adjusted to pH4.0could produce the citric acid at a yieldof 73.59% based on the sugar cons-umed. When the spores were inoculatedat a concentration of 2.4ml/100ml inthe distilled slop with the addition of0.25g/1 , 4% methanol,0.05% Ferrocyanide and 6% sucrose atpH4/0. the yield of citric acid couldreach to 80.29% based on the sugarconsumption and 78.15% on the sugaraddition. After the citric acid fermen-tation, the BOD of distilled slop couldreduce 81.55%.
• 中文關鍵字： --
• 英文關鍵字： --