- 作者: Bor-Show Tzang; Chun-Ching Chiu; Chun-Chou Tsai; Yi-Ju Lee; I-Jung Lu; Jing-Yu Shi; Tsai-Ching Hsu
- 作者服務機構: Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background: Activity of secreted phospholipase A (sPLA2) has been implicated in a
wide range of cellular responses. However, little is known about the function of
human parvovirus B19-VP1 unique region (VP1u) with sPLA2 activity on
macrophage.
Methods: To investigate the roles of B19-VP1u in response to macrophage,
phospholipase A2 activity, cell migration assay, phagocytosis activity,
metalloproteinase assay, RT-PCR and immunoblotting were performed.
Results: In the present study, we report that migration, phagocytosis, IL-6, IL-1d
mRNA, and MMP9 activity are significantly increased in RAW264.7 cells by
B19-VP1u protein with sPLA2 activity, but not by B19-VP1uD175A protein that is
mutated and lacks sPLA2 activity. Additionally, significant increases of
phosphorylated ERK1/2 and JNK proteins were detected in macrophages that were
treated with B19-VP1u protein, but not when they were treated with
B19-VP1uD175A protein.
Conclusions: Taken together, our experimental results suggest that B19-VP1u with
sPLA2 activity affects production of IL-6, IL-1d mRNA, and MMP9 activity,
possibly through the involvement of ERK1/2 and JNK signaling pathways. These
findings could provide clues in understanding the role of B19-VP1u and its sPLA2
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enzymatic activity in B19 infection and B19-related diseases. - 中文關鍵字: --
- 英文關鍵字: --