第8卷‧第11期,
198011
, pp. 1055-1066
紋白蝶顆粒體病毒之組織病理學研究
- 作者:
蘇智勇; 嚴奉琰; 何鎧光
- 作者服務機構:
臺灣香焦研究所; 國立臺灣大學植物病蟲害學研究所
- 中文摘要:
紋白蝶顆粒體病毒可感染真皮、脂肪體及氣管被膜細胞,故屬於嗜多器官病(polyorgano-tropic disease)。病毒由核開始感染及複製,然後擴散到細胞質,所以被視為細胞核型的病毒。 光學顯微鏡觀察組織病理變化:感染初期核稍微膨大,接著核繼續膨脹,細胞亦隨之增大。感染中期,細胞緊縮為固縮核(pycnotic nuclei),進而核膜破裂。末期,網狀組織散佈於整個細胞質,細胞核質與細胞質混淆不清,且空胞完全消失,充滿著莢膜體(capsule);終致細胞完全破裂,釋放出莢膜體,充滿體腔。 紋白蝶的顆粒體病毒之莢膜體為卵形或不正卵形,長約為 300 nm,寬約為 200 nm。病毒粒子為桿狀,大小約為 280 x 50 nm。 電子顯微鏡觀察,顯示病毒粒子由病毒子座(virogenic stroma)釋放出來,然後擴散到細胞質。病毒在感染細胞核內複製和發育,起初為球形,接著變為桿狀,最後,病毒粒子為蛋白質所包繞而成莢膜體。病毒粒子及包著病毒粒子的蛋白質外殼,係由內質網之網狀組織所衍生的,故網狀組織可認為是病毒子座。
- 英文摘要:
The granulosis of Pieris rapae is a poly-organotropic disease, i. e., the virus may infectfat body, epidermis and tracheal matrix; andits replication starts from nucleus and thenspreads to cytoplasm. Optical microscopic observations on histo-pathology changes in P. rapae are describedbelow: the nuclear materials of fat body cells,especially the nucleolus, seemed to break up andto condense into irregular masses 24-48 hr afterinoculation. Pycnotic nuclei with condensedchromatins may be seen in the center. Sometimesthe heavily stained nuclear materials presenteda continuous or partial form near the nuclearmembrane. The nuclei continued to hypertrophyand their membranes became barely visible 72 hrafter inoculation, therefore, the whole cell in-creases in size. As a result of enlargementof the nucleus or, more probably, disintegrationof the nuclear membrane, the nuclear and cy-toplasmic elements were intermingled 96 hr afterinoculation. At 144 hr after inoculation, thehemocoele was filled with capsules which werereleased by rupture of infected fat bodyand other susceptible tissues. Histopathologicalchanges occurred in epidermis and tracheal ma-trix in the same manner as fat body cells.However, the progress of infection in the epi-dermal cells and tracheal matrix cells tended tobe more uniform than the fat body cells. The capsules of this virus were ovoid orsubovoid measuring 300×200 nm. The virusparticles were rod-shaped, ca. 280×50 nm. The virus multiplied and developed withinthe nuclei of infected cells, first as spheres andthen as rods, finally, each virus became occludedby protein deposition to form a capsule. Thevirus rods, capsulse, partial of encapsulation,intimate membrane of virus, developmentalmembrane of virus, multimembranous structureand ring-shaped structure embedded in the fatbody cells. Virogenic stroma showed on themicrographs 72 and 96hr after inoculation, butthey disappeared 120hr after inoculation withgranulosis virus of P. rapae.
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