- 作者: 韓素琴,劉二保,李華
- 作者服務機構: Institute of Analytical Science, Northwest University, Xi’an 710069, P. R. China
- 中文摘要: Using the hemin-H2O2-Na2CO3-NaOH chemiluminescence (CL) system, the study on the photodecomposition behavior of hemin under ultraviolet light and solar light were carried out and the determination of hemin was developed coupled with simple flow injection technique. The results showed that the decomposition reaction of hemin in different light irradiations corresponded with a first-order reaction. And then the determination of hemin was completed by the CL emission from the reaction of hemin with H2O2 in aqueous carbonate. The linear range was 2.2 ’ 10-10 to 6.88 ’ 10-7 M and the detection limit was 2.2 ’ 10-11 M (S/N = 3). The relative standard deviation (RSD) was 2.82% for ten independent detections of 1.72 ’ 10-8 M hemin. As a preliminary application, the proposed method was successfully applied for the analysis of hemin in pharmaceutical formulations and animal blood with a recovery of 96~108%. A possible CL mechanism of the present system was discussed, and free radicals were suggested to be involved in this reaction.
- 英文摘要: --
- 中文關鍵字: Flow-injection; Chemiluminescence; Hemin; Photodecomposition kinetics; Hydrogen peroxide.
- 英文關鍵字: --