- 作者: Ing-Cherng Guo; Meng-Chun Shih; Hsin-Chieh Lan; Nai-Chi Hsu; Meng-Chun Hu; Bon-chu Chung
- 作者服務機構: 1 Department of Veterinary Medicine, College of Bio-Resources and Agriculture, National Taiwan University, Taipei, Taiwan; ; 2 Institute of Molecular Biology, Academia S璯ica, Nankang, Taipei, 115, Taiwan; ; 3 Institutes of Molecular and Cellular Biology and Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan; ; 4 Graduate Institute of Physiology, National Taiwan University College of Medicine, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: The CYP11A1 gene encodes the cholesterol side-chain cleavage enzyme, also termed cytochrome P450scc, which catalyzes the conversion of cholesterol to pregnenolone in the first step of steroid biosynthesis in mitochondria. The adrenal- and gonad-selective, hormonally and developmentally regulated expression of CYP11A1 is principally driven by its 2.3 kb promoter. Multiple trans-acting factors like SF-1, Sp1, AP-2, TReP-132, LBP-1b, LBP-9, AP-1, NF-1, and Ets control CYP11A1 transcription either through DNA-protein interaction with their specific cis-acting elements or through protein-protein interaction between each other, wherein SF-1 plays a central role in adrenals and testes. In addition to binding with its proximal and upstream motifs, SF-1 also physically interacts with TFIIB, CBP/p300, TReP-132, and c-Jun/AP-1 to specifically transmit the regulatory signals of cAMP. Other factors like Sp1 family members, AP-2, and LBP-1b/LBP-9 may be other factors that play a role in CYP11A1 transcription, particularly in placental cells. The TATA sequence could also contribute to tissue-specificity and hormonal regulation of CYP11A1 transcription. This article reviews recent studies focusing on adrenals and gonads.
- 中文關鍵字: --
- 英文關鍵字: CYP11A1, P450scc, cholesterol side-chain cleavage enzyme, SF-1, adrenal, steroidogenesis, steroid, gonad, transcription