- 作者: 陳水田 ; 張忠和 ; 林強生 等
- 作者服務機構: 台灣大學生化科學研究所
- 中文摘要: Procedures for preparing C-terminal free peptides from hydrolysis of its corresponding methyl or benzyl esters catalyzed by alkaline protease has been developed. N-protected peptides having side-chain ester protecting groups or successive hydrophobic amino acid residues in its sequence are hydrolyzed selectively at C-terminal only and leave other bonds (.beta. and .gamma.-ester or peptide bonds) intact. Compounds which cause a side reaction in base mediated saponification could be hydrolyzed safely by this procedure. Products of this hydrolysis are useful intermediates for fragments coupling in the solid phase peptide synthesis.
- 英文摘要: --
- 中文關鍵字: Selective Hydrolysis; Peptide; Amino Acid; Coupling; Enzyme
- 英文關鍵字: 選擇性水解;胜;胺基酸;耦合;酵素