- 作者: Ping-Chia Li; Sheng-Chung Li; Yuan-Ju Lin; Jin-Tung Liang; Chiang-Ting Chien; Chen-Fu Shaw
- 作者服務機構: 1 Department of Biological Science, National Sun Yat-Sen University, Kaohsiung, ROC; ; 2 Department of Electronics Engineering, Yung-Ta Institution of Technology and Commerce, Ping-Don, ROC;; 3 Department of Family Medicine, National Cheng-Kung University Hospital, Tainan, ROC; ; 4 Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, ROC; ; 5 Department of Medical Research, National Taiwan University Hospital and National Taiwan University College of Medicine, 7 Chung-Shan S. Road, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: Electrical stimulation of efferent thoracic vagus nerve (TVN) evoked neurogenic inflammation in respiratory tract of atropine-treated rats by an undefined mechanism. We explored whether efferent TVN stimulation via substance P facilitates neurogenic inflammation via action of nuclear factor-κb (NF-κB) activation and reactive oxygen species (ROS) production. Our results showed that increased frequency of TVN stimulation concomitantly increased substance P-enhanced hypotension, and bronchoconstriction (increases in smooth muscle electromyographic activity and total pulmonary resistance). The enhanced SP release evoked the appearance of endothelial gap in silver-stained leaky venules, India-ink labeled extravasation, and accumulations of inflammatory cells in the respiratory tract, contributing to trachea plasma extravasation as well as increases in blood O2 and H2O2 ROS amount. L-732138 (NK1 receptor antagonist), SR-48968 (NK2 receptor antagonist), dimethylthiourea (H2O2 scavenger) or catechins (O2- and H2O2 scavenger) pretreatment reduced efferent TVN stimulation-enhanced hypotension, bronchoconstriction, and plasma extravasation. Increased frequency of TVN stimulation significantly upregulated the expression of nuclear factor-κB (NF-κB) in nuclear protein and intercellular adhesion molecule-1 (ICAM-1) in total protein of the lower respiratory tract tissue. The upregulation of NF-κB and ICAM-1 was attenuated by NK receptor antagonist and antioxidants. In conclusion, TVN efferent stimulation increases substance P release to trigger NF-κB mediated ICAM-1 expression and O2- and H2O2 ROS production in the respiratory tract.
- 中文關鍵字: --
- 英文關鍵字: intercellular adhesion molecule-1, nuclear factor-κB, reactive oxygen species, substance P, thoracic vagus nerve