- 作者: Amita Sharma, Ravi Vasanthapuram, Manjunatha M Venkataswamy and Anita Desai
- 作者服務機構: 1.Department of Neurovirology, National Institute of Mental Health and Neurosciences, 560029, Bengaluru, India
- 中文摘要:
- 英文摘要:
Background
Very few studies have identified receptor molecules for dengue virus (DENV) on neural cells. This study was designed to identify putative receptor/(s) involved in entry of DENV-3 in human neural cells of various lineages; neuronal-SH-SY5Y, astroglial-U-87 MG and microglial-CHME-3 cells.
Result
Virus overlay protein binding assay, LC-MS/MS and SEQUEST identified prohibitin1/2 (PHB1/2) as interacting proteins on SH-SY5Y, CHME-3, and U-87 MG cells. Infection inhibition and siRNA assays confirmed the role of PHB1/2 in the entry of DENV-3 into SH-SY5Y and CHME-3 cells but not in U-87 MG cells. Indirect immunofluorescence and flow-cytometry demonstrated the presence of PHB1/2 on the surface of SH-SY5Y and CHME-3 cells. Co-immunoprecipitation and Western blot, as well as double labelling, reconfirmed the interaction between PHB1/2 and DENV-3 EDIII protein.
Conclusion
These observations together for the first time indicate that PHB1/2 may serve as a putative receptor for DENV-3 in SH-SY5Y and CHME-3 cells. The study provided insights into DENV-3 and neural cell interactions. - 中文關鍵字:
- 英文關鍵字: Dengue virus serotype-3, Neural cells, SH-SY5Y, CHME-3 cells, Receptor/ interacting proteins, Prohibitin