- 作者： A-Mei Huang; Tai-Jay Chang; Wen-Long Cho; Chen-Kung Chou
- 作者服務機構： Institutes of ; a Genetics and; b Parasitology, National Yang-Ming University, and ; c Department of Medical Research, Veterans General Hospital, Shih-Pai, Taipei and ; d Division of Molecular and Genomic Medicine, National Health Research Institutes, Nan-Kang, Taipei, Taiwan, ROC
- 中文摘要： --
- 英文摘要： Protein kinases play an important role in the signaling pathway of growth factors in most of the higher organisms. During the study of protein kinase profiles of mosquitoes using RT-PCR and degenerate primers for consensus catalytic domain motifs to amplify protein kinase genes, we have noticed that a novel mosquito kinase, AaPK-38, shares a stretch of amino acids identical to the corresponding domain in Tousled gene of Arabidopsis thaliana that is required for leaf and flower development. A 2.1-kb cDNA encoding human HsHPK gene, which is a homolog of AaPK-38, was isolated from human testis cDNA library. This cDNA contains an open reading frame of 563 amino acids, with a complete kinase domain in its carboxyl terminus. The expressed Flag-tagged HsHPK was shown to have kinase activity based on in vitro auto-phosphorylation. Northern blot analysis revealed that human HsHPK mRNA is most abundant in testes, much less in heart and skeletal muscle and almost undetectable in liver and lung. Finally, we found that the expression of HsHPK in 4 out of 6 human hepatoma tissues is much higher than that in the adjacent normal counterpart. This result suggests HsHPK may play a role in the development of human hepatoma.
- 中文關鍵字： --
- 英文關鍵字： Protein kinase ; Human liver cancer ; Tousled gene
