- 作者: Chi-Jung Huang a, Jeou-Yuan Chen b
- 作者服務機構: a Graduate Institute of Life Sciences, National Defense Medical Center, b Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: the CRS in their sequences, as they contain theCG (N) CG motif. The binding of IE2-p86 to these twodistal CRS-like sequences was further confirmed byDNase I footprinting analysis and electrophoretic mobili-ty shift assay. Promoter activity analysis in the transientexpression system suggested that these two cis ele-ments act functionally as IE2-p86-responsive repressivesequences to cooperate with the CRS to suppress MIEPexpression.Human cytomegalovirus (HCMV) is a ubiquitous humanpathogen that is the leading viral cause of birth defectsand also causes significant morbidity and mortality inimmunosuppressed individuals. The immediate early(IE) genes, IE1-p72 and IE2-p86, are the first HCMV genesexpressed after infection under the control of a strongtranscriptional enhancer-promoter, the major IE promot-er (MIEP). Gene expression mediated by the predomi-nant IE2-p86 is believed to be essential for the progres-sion of viral production, as well as for the developmentof HCMV-associated pathogenesis. To gain further un-derstanding of the transcriptional activity of IE2-p86, weattempted to isolate its downstream target genes withinthe HCMV genome. By a modified approach coupling themethods of cyclic amplification and selection of targetsand selection and amplification of binding sites, severalHCMV genomic fragments were identified based on theirability to bind to IE2-p86. Two additional IE2-p86-respon-sive elements other than the cis-repressive sequence(CRS) were identified within the MIEP and were termed-240 and-170 boxes. These two cis elements resemble
- 中文關鍵字: --
- 英文關鍵字: Human cytomegalovirus, Major immediate early promoter, Immediate early protein 2, cis repression sequence