- 作者: Minyoung Kong; Younyoung Kim; Chaeyoung Lee
- 作者服務機構: Ilsong Institute of Life Science, Hallym University, South Korea
- 中文摘要: --
- 英文摘要: LIGHT (homologous to Lymphotoxins, exhibitsInducible expression, and competes with herpes simplexvirus Glycoprotein D for Herpes virus entry mediator, areceptor expressed by T lymphocytes) is implicated in theinflammation by disrupted T cell homeostasis, primarily ata transcriptional level. We investigated the association ofLIGHT promoter with ischemic stroke and vasculardementia induced by such inflammation. We determinedtranscription factor binding sites altered by promoter SNPsusing transcription factor prediction programs. Six commonhaplotypes composed of the selected SNPs (C-770T,G-607T, G-543A, and A-399G) were used for the assay ofreporter activity. The most frequent haplotype construct,CGGA, induced the highest luciferase activity. The haplotypeTTGA showed the lowest expression with 0.39-foldactivity (P\0.001) of CGGA. The substitution from C toT at the locus of C-770T (TGGA) decreased the reporteractivity by 47% (P\0.001). The SNPs and haplotypeswere further investigated to see their association withischemic stroke and vascular dementia in 455 controls and478 patients. Significant association with vascular dementiawas shown in the allele T of C-770T (odds ratio[OR] = 1.54; P\0.05) and the haplotype TTGA (OR =10.59; P\0.05) in females. We concluded that the alleleT of C-770T and the haplotype TTGA of the promoterSNPs in LIGHT gene might decrease the expression ofLIGHT and subsequently increase the susceptibility tovascular dementia in females.
- 中文關鍵字: --
- 英文關鍵字: Estrogen; Genetic risk factor; Inflammation; Vascular dementia