- 作者: Francis J. Cahill; Elizabeth A. Ellenberger; Janet L. Mueller; Leon F. Tseng; Raymond M. Quock
- 作者服務機構: Department of Biomedical Sciences, University of Illionis College of Medicine at Rockford, III., USA; Department of Anesthesiology, Medical College OF wisconsin, Milwaukee, Wisc., USA
- 中文摘要: --
- 英文摘要: Previously it was demonstrated that nitrous oxide anti-nociception in the mouse abdominal constriction test ismediated by x-opioid receptors. Since nitrous oxide isthought to cause the neuronal release of endogenousopioid peptide to stimulate opioid receptors, this studywas designed to identify the opioid peptides involved,especially in the spinal cord, by determining whethernitrous oxide antinociception can be differentially inhib-ited by intrathecally (i.t.) administered antisera to differ-ent opioid peptides. Male NIH Swiss mice were pre-treated i.t. with rabbit antisera to opioid peptides thenexposed 24 h later to one of three different concentra-tions of nitrous oxide in oxygen. Dose-response curvesconstructed from the data indicated that the antinocicep-tive effect of nitrous oxide was significantly antagonizedby antisera to various dynorphins (DYNs) and methio-nine-enkephalin (ME), but not by antiserum to (3-endor-phin (3-EP). The AD50 values for nitrous oxide antinoci-ception were significantly elevated by antisera to DYNsand ME but not 3-EP. These findings of this study supportthe hypothesis that nitrous oxide antinociception in themouse abdominal constriction test involves the neuronalrelease of DYN and ME in the spinal cord.
- 中文關鍵字: --
- 英文關鍵字: --