- 作者: Zhenqiang Zhao; Zhibin Chen; Xiubo Zhao; Fang Pan; Meihua Cai; Tan Wang; Henggui Zhang; Jian R Lu; Ming Lei
- 作者服務機構: Department of Neurology, Affiliated Hospital, Hainan Medical College, Haikou, Mainland China.
- 中文摘要: --
- 英文摘要:
Background: It is of growing interest to develop novel approaches to initiate differentiation of mesenchymal stem cells (MSCs) into cardiomyocytes. The purpose of this investigation was to determine if Sphingosine-1-phosphate (S1P), a native circulating bioactive lipid metabolite, plays a role in differentiation of human umbilical cord mesenchymal stem cells (HUMSCs) into cardiomyocytes. We also developed an engineered cell sheet from these HUMSCs derived cardiomyocytes by using a temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) cell sheet technology.
Methods: Cardiomyogenic differentiation of HUMSCs was performed by culturing these cells with either designated cardiomyocytes conditioned medium (CMCM) alone, or with 1 muM S1P; or DMEM with 10% FBS + 1 muM S1P. Cardiomyogenic differentiation was determined by immunocytochemical analysis of expression of cardiomyocyte markers and patch clamping recording of the action potential.
Results: A cardiomyocyte-like morphology and the expression of alpha-actinin and myosin heavy chain (MHC) proteins can be observed in both CMCM culturing or CMCM+S1P culturing groups after 5 days' culturing, however, only the cells in CMCM+S1P culture condition present cardiomyocyte-like action potential and voltage gated currents. A new approach was used to form PIPAAm based temperature-responsive culture surfaces and this successfully produced cell sheets from HUMSCs derived cardiomyocytes.
Conclusions: This study for the first time demonstrates that S1P potentiates differentiation of HUMSCs towards functional cardiomyocytes under the designated culture conditions. Our engineered cell sheets may provide a potential for clinically applicable myocardial tissues should promote cardiac tissue engineering research. Key words: umbilical cord mesenchymal stem cells, sphingosine-1- phosphate, engineered cell sheets
- 中文關鍵字: --
- 英文關鍵字: umbilical cord mesenchymal stem cells; sphingosine-1- phosphate; engineered cell sheets