• 作者： Suen, Shing-Yi; Chang, Yi-Shou
• 中文摘要： 本研究探討在使用不同固體基材為介質下lysozyme與bovine serum albumin對配位基Cibacron Blue 3GA之吸附與脫附行為，所用固體介質為層析顆粒與圓片薄膜。實驗結果顯示：lysozyme與BSA均可吸附至層析顆粒，但僅有lysozyme被吸附至圓片薄膜上。此外，吸附方向與脫附方向所得之等溫線並不重合，雖然他們二者均代表定溫下達到平衡狀態時蛋白質被吸附量與溶液濃度間之關係。同樣地，脫附方向之速率實驗亦呈現與吸附方向之速率實驗不同之結果。另一方面，本研究利用適當之數學模式來模擬實驗結果，所得模擬結果吻合實驗數據。
• 英文摘要： The adsorption and desorption properties of lysozyme and bovine serum albumin to immobilized Cibacron Blue 3GA using different solid supports, gel beads and membrane discs, are investigated in this study. The experimental results show that both lysozyme and BSA were adsorbed onto the gel beads, but only lysozyme can be bound to membranes. Moreover, the adsorption and desorption isotherms were different even though they both represent the relationship between the adsorbed amount and the solution concentration at equilibrium under a certain temperature. The desorption kinetics are also found distinct from the adsorption kinetics. On the other hand, effective models were used to fit the experimental results. The modeling results are in good agreement with the experimental data.
• 中文關鍵字： 溶菌; 白蛋白; 汽巴藍; 蛋白質; 親和性支持
• 英文關鍵字： Lysozyme; Albumin; Cibacron Blue; Protein; Affinity Support