- 作者: Maria A Macri, Nicola D'Alessandro, Camillo Di Giulio, Patrizia Di Iorio, Silvano Di Luzio, Patricia Giuliani, Ennio Esposito, Mieczyslaw Pokorski
- 作者服務機構: Department of Experimental Medicine and Pathology, “La Sapienza” University, Rome and S. Lucia Foundation, Rome, Italy
- 中文摘要: --
- 英文摘要:
Background: Both hypoxia and hyperoxia, deregulating the oxidative balance, may play a role in
the pathology of neurodegenerative disorders underlain by cerebral ischemia. In the present
study, quantitative proton magnetic resonance spectroscopy was used to evaluate regional
metabolic alterations, following a 24-hour hypoxic or hyperoxic exposure on the background of
ischemic brain insult, in two contrasting age-groups of rats: young – 3 months old and aged – 24
months old.
Methods: Cerebral ischemia was induced by ligation of the right common carotid artery.
Concentrations of eight metabolites (alanine, choline-containing compounds, total creatine, γ-
aminobutyric acid, glutamate, lactate, myo-inositol and N-acetylaspartate) were quantified from
extracts in three different brain regions (fronto-parietal and occipital cortices and the
hippocampus) from both hemispheres.
Results: In the control normoxic condition, there were significant increases in lactate and myoinositol
concentrations in the hippocampus of the aged rats, compared with the respective values
in the young ones. In the ischemia-hypoxia condition, the most prevalent changes in the brain
metabolites were found in the hippocampal regions of both young and aged rats; but the effects
were more evident in the aged animals. The ischemia-hyperoxia procedure caused less dedicated
changes in the brain metabolites, which may reflect more limited tissue damage.
Conclusions: We conclude that the hippocampus turns out to be particularly susceptible to
hypoxia overlaid on cerebral ischemia and that old age further increases this susceptibility. - 中文關鍵字: --
- 英文關鍵字: --