- 作者: 陳煒富; 簡美美; 染善居; 王敦梅
- 作者服務機構: 國立臺灣大學農學院獸醫學系
- 中文摘要: BHK-21細胞感染新城雞瘟病毒(NDV)後以5μg/ml之Cytochalasin B(CB)藥物處理,以相位、間接螢光及超薄切片電子顯微鏡觀察細胞微細構造之變化。結果,CB處理之病毒力價及抗actin螢光強度減低,同時細胞膜之破損及對NDV特異性抗原之鐵蛋白標示免疫抗體附著性損失。
- 英文摘要: The microfilament structures of Newcastle disease virus (NDV)-infected BHK-21 cells were studiedin the presence (5 μg/ml) or absence of cytochalasin B (CB) by means of phase contrast, indirect immuno-fluorescence and thin-section immunoelectron microscopy. The results indicated that CB treatment notonly impaired virus infections titers and antiactin fluorescence strength but also disrupted cytoplasmicmembrane and untagged ferritin-conjugated antibody on the surface of NDV specific antigens.
- 中文關鍵字: Newcastle disease virus; cytochalasin B; cytoskeleton; labeled ferritin antibodies
- 英文關鍵字: --