- 作者: Frank E. Shafer ; Boro Dropulic; Cindy Ely; Catherine A. Schaefer; Diana Freas; H. Ewa Witkowska; Alan N. Schechter; Constance T. Noguchi; Michael Dewey; StefanKarlsson
- 作者服務機構: a Department of Hematology/Oncology and; Research Institute of Children's Hospital; Oakland, Calif., b Laboratory of Molecular Microbiology, NIAID, c Developmental and Metabolic Neurology; Branch, NINDS, d Laboratory of Chemical Biology, ; NIDDK, NIH, Bethesda, Md., and; e Department of Biology, University of; South Carolina, Columbia, S.C., USA
- 中文摘要: --
- 英文摘要: Two transgenic lines of mice were produced which contained the βS Antilles_and α2-hemoglobin genes trandemly coupled to the `micro' locus control region (μLCR). The μLCRβ SAntilles-hemoglobin transgenic mice expressed high levels of α2-hemoglobin while βS Antilles_heemoglobin expression was virtually undetecta- ble. Abundant α2-hemoglobin protein was observed in the blood of transgenic mice, while βSAntilles hemoglobin chains could not be deteced. Transgenic red blood cells had substantially decreased sensitivity to osmotic lysis. Attempts to produce homozygotes containing the transgene were unsuccessful. The phenotype of these mice closely resembles that of β-thalassemic mice. The μLCRβSAntilles_α2 transgenic mice demonstrate that if the μLCR is coupled to the βSAntilles_and α2-hemoglobin genes in tandem, only the distal α2-hemoglobin gene is selected for expression to significant levels in adult mice. These results support a reciprocally competitive model for LCR-hemoglobin developmental switching.
- 中文關鍵字: --
- 英文關鍵字: Transgenic mice; Locus control region; α-Globin; β-Globin; β-Thalassemia; Sickle cell anemia