- 作者: Lein-Tuan HOU ; Cheing-Meei LIU ; Yi-Jane CHEN ; Man-Ying WONG ; Jinkun CHEN ; Kun-Chee CHEN
- 中文摘要: The purpose of this study was to investigate the effect of acetic acid-extracted bone proteins on human periodontal ligament fibroblasts (hPF) with respect to mitogenic and cell attachment promoting activity. Alveolar bone was harvested from healthy donors and subjected to 0.5 M acetic acid extraction, dialysis and lyophilization, and gel filtration. Promotion of cell attachment and stimulation of DNA synthesis by the crude extract and gel-filtrated fractions were studied in cultured hPF. Many protein components, varying in molecular weight from 10-14 to 120 kDa, were detectable in 10% SDSPAGE of the extract. Gel filtration of bone extract disclosed four fractions with molecular weights of 55, 34, 29 and 19-20 kDa. Both the 34 and 55 kDa fractions at a concentration of 5 .mu.g/ml, but not the 29- or 19-20 kDa fractions, were found to promote cell attachment while only the 55 kDa fraction (5 .mu.g/ml) stimulated DNA synthesis of hPF. Both mitogenic activity and the promotion of the cell attachment by gel-filtrated active fractions were resistant to thermal treatment (70.degree.C) and pH (4 - 8) changes. These findings suggest that acetic acid extract of alveolar bone may contain components which are capable of modulating cell attachment and mitogenesis of hPF.
- 英文摘要: --
- 中文關鍵字: gel filtration, alveolar bone extract, periodontal ligament fibroblast, cell attachment, mitogenicity
- 英文關鍵字: --