• 作者： Chung, T.W.; Hwung, G.H.; Chen, W.K.et al.
• 中文摘要： 本研究探討血紅素包覆型替代血液(LEH)經血漿培養,再經由cone/plate黏度計在不同剪應力下作用2分鐘後的穩定度分析。其結果顯示,血紅素及包覆之脂質滲出量因血漿與LEH之比值增加,培養時間之增長及剪切率之增高而增大。同時,經血漿培養或不同剪應力作用後之LEH,其釋出之血紅素及脂質皆呈現強烈之正相關。另外經比較,我們認為剪應力是影響LEH不穩定的重要因子。
• 英文摘要： The released concentration of hemoglobin ( [ Hb ] ) or phosphatidylcholine ( [ PC ] ) from liposome encapsulated hemoglobin suspensions (LEH) after incubated in plasma has been investigated under different shear force. After the sample exposed to a rotational viscometer for 2 minutes, released [ Hb ] and [ PC ] were determined by a spectrophotometric assay. The results showed that the quantities of Hb and PC released increased with increasing volume ratio of plasma to LEH, longer time period of plasma incubation and higher shear force applied to LEH. There was a strong correlation between released [ Hb ] and [ PC ] after LEH was incubated with different volume ratio of plasma (e.g., [ Hb ] =1.763 [ PC ] +0.015, R=0.99, for 8 part of LEH to one part of plasma). Furthermore, strong correlations between [ Hb ] and [ PC ] also observed for LEH suspensions under different shear rates. The results of the study also suggested that shear force is an important factor to cause the releases of PC and Hb from LEH.
• 中文關鍵字： 血漿; 血紅素; 剪應力; 血紅素包覆型替代血液; 黏度
• 英文關鍵字： Plasma; Hemoglobin; Shear Force; Liposome Encapsulated Hemoglobiun; Viscosity