- 作者: Timothy Kudinha & Fanrong Kong
- 作者服務機構: 1.NSW Health Pathology, CIDMLS, Westmead Hospital, Westmead, NSW, 2145, Australia 2.NSW Health Pathology, Regional and Rural, Orange Hospital, Orange, NSW, Australia 3.School of Biomedical Sciences, Charles Sturt University, Leeds Parade, Orange, NSW, 2800, Australia
- 中文摘要:
- 英文摘要:
Background: Escherichia coli sequence type (ST)131 is an important urinary tract pathogen, and is responsible
for considerable healthcare-associated problems and costs worldwide. A better understanding of the factors that
contribute to its rapid worldwide spread may help in arresting its continual spread. We studied a large collection of
fecal and urinary E. coli ST131 and E. coli non-ST131 phylogenetic group B2 isolates, from women, men and children, in
regional NSW, Australia.
Results: We found out that there was a step up in ST131 prevalence (and possibly in virulence) from fecal to clinical
(urinary) isolates in general, and specifcally among ciprofoxacin resistant isolates, in the 3 host groups. Furthermore,
our results revealed that the inferred virulence potential of the ST131 isolates (as measured by VF gene scores) was
much higher than that of non-ST131 phylogenetic group B2 isolates, and this was much more pronounced amongst
the urinary isolates. This fnding suggests presence of possible E. coli phylogenetic B2 subgroups with varying levels of
virulence, with ST131 being much more virulent compared to others. A strong association between ST131 and fuoroquinolone (FQ) resistance was also demonstrated, suggesting that FQ use is related to ST131 emergence and spread.
Specifcally, about 77% of ST131 isolates from women and men, and 47% from children, were extended spectrum βlactamase (ESBL) producers. Moreover, FQ resistant ST131 ESBL isolates on average harbored more VF genes than all
other isolates.
Conclusions: The strong association between ST131 prevalence and FQ resistance amongst the studied isolates
suggests that FQ use is related to ST131 emergence and spread. Furthermore, our results demonstrate that FQ resistance and a plurality of VF genes can exist together in ST131, something that has traditionally been regarded as being
inversely related. This may partly contribute to the emergence and worldwide spread of ST131. - 中文關鍵字:
- 英文關鍵字: Escherichia coli, Virulence genes, Antibiotic resistance