- 作者: Yi-Ming A. Chen Wei-Yu Rey Yu-Ching Lan Shu-Fen Lai Yu-Chuan Huang Shiow-lng Wu Tze-tze Liu Kwang-Jen Hsiao
- 作者服務機構: AIDS Prevention and Research Center, Institute of Public Health, School of Medicine, Genome Research Center, National Yang-Ming University, Taipei Venereal Disease Control Institute, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要:
Human immunodeficiency virus type 1 (HIV-1) Vpu pro-
tein promotes both extracellular release of viral particles
and degradation of CD4 in the endoplasmic reticulum.
The correlation of anti-Vpu antibody (Ab) reactivity to
Vpu and AIDS disease progression was studied in 162
HIV-1/AIDS patients after they had received highly active
antiretroviral therapy (HAART) for 1 year. Anti-Vpu Ab
reactivity was analyzed by Western blot using a recombi-
nant Vpu protein. Results showed that at baseline (prior
to initiation of HAART), 31.5% of patients (51/162) had
anti-Vpu Ab. The proportion of anti-Vpu Ab in patients
with CD4 counts ≧500, 200-500 and<200/mm3 were
40.6, 34.7 and 14.3%, respectively (x2 test, p<0.05). In
addition, decreasing levels of anti-Vpu Ab reactivity were
significantly correlated with increasing levels of HIV-1
viral load. After receiving HAART for 1 year, 7 of 111 anti-
Vpu Ab-negative patients (6.3%) seroconverted (-→+
group) and 8 of 51 anti-Vpu Ab-positive (15.7%) patients
became negative (+→-group) . Among 104 anti-Vpu Ab-
negative patients, 40 were selected for analysis of the
vpu gene. All of them had an intact vpu gene. Patients
were further divided into four groups according to their
anti-Vpu Ab serostatus and anti-HIV-1 Ab was measured.
The results showed that only the anti-Vpu Ab serocon-
verted group (-→+) had increased serum levels of anti-
HIV-1 Abs after 1 year of HAART, while the other three
groups (+→+, -→-and+→-) had decreased serum
levels of anti-HIV-1 Abs after 1 year of HAART (p<0.05).
In conclusion, the presence of anti-Vpu Ab is associated
with improved prognosis following HIV-1 infection, and
seroconversion of anti-Vpu Ab in patients on HAART
indicates significant recovery of immunity. - 中文關鍵字: --
- 英文關鍵字: Anti-Vpu antibody. lgG subclasses. vpu gene. Viral load. Total lgG. Disease progression. Highly active antiretroviral therapy. AIDS