• 作者： 許織雲; 葉詩雅; 張麗姿
• 作者服務機構： 國防防醫學院生物形態學系
• 中文摘要： 牛蛙變態期蚪蝌卵巢在體外培養6小時可藉放射免疫測定法（RIA，知其能分泌雌二醇(E)。將卵巢剪碎為lx1 x 2mm，30 mg置於一試管中，試管中含1 ml之培養液。此試管於20°C下振盪，兩小時後分離其培養液。如此重覆三次，共收集3 ml之培養液。用乙醚萃取類固醇，再用RIA方法測定。得知變態期蚪蝌卵巢，一如成熟者然，可釋放E於培養液中。此離體卵巢可作為日後分析在生理情況下及實驗條件時之E分泌量。
• 英文摘要： An in vitro system for incubating ovaries of metamorphosing tadpoles of Rana catesbeiana to secreteestradiol (E) is established with the aid of radioimmunoassay (RIA). 30 mg of ovaries cut into lx2x2 mmin size were put in a plastic tube containing one ml of the incubation medium for two hours at 20°C. Themedium was collected. The ovaries were incubated in another one ml of new medium for two hours andthe medium collected again. This process was repeated for the third time. Three ml of the medium werepooled at the end of six hours. Ether extraction of the medium was analyzed for E with RIA. The resultshowed that immature ovaries of metamorphosing tadpoles had the capacity, as that of the mature ones,to secrete E into the medium. This system is of significance to work on the immature ovaries of tadpolesunder physiological and experimental conditions.
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