• 作者： 王慧如; 蔡元奮
• 作者服務機構： 國立臺灣大學醫學院生理學科
• 中文摘要： 本實驗旨在探討雄鼠尿液的刺激對雌鼠副嗅球內正腎上腺素濃度的變化，並觀察是否由於雌鼠年齡的差異，因而形成不同的反應。實驗動物為年輕（3至4個月）及年老（24至25個月）Sprague-Dawley雌鼠，俟卵巢切除一個月之後，以雌二醇膠囊種植於其皮下。一週後將上述處理之雌鼠一側的鋤鼻器摘除，而再經過一週，在中午12時開始給予雄鼠尿液的嗅覺刺激。在接受尿液刺激之前及其後經過0，1，2，3小時，斷頭犧牲動物，以刺孔方式取出兩側副嗅球，利用高壓液相色層分析法測量副嗅球內正腎上腺素的含量。實驗的結果顯示：在14時年輕雌鼠鋤鼻器摘除側的副嗅球內正腎上腺素濃度較其在12時者明顯降低，而年老雌鼠鋤鼻器摘除側副嗅球內正腎上腺素的濃度，在相同時間內，卻無明顯的變化。連續接受雄鼠尿液刺激2小時之後（在14時），年輕雌鼠鋤鼻器未摘除側副嗅球內正腎上腺素的濃度較其未接受鼠尿刺激前（在12時）明顯增高，而在年老雌鼠則未發現有相似的反應。以上的結果說明雌鼠副嗅球內的正腎上腺素性功能可能係隨著年齡的增加而改變。
• 英文摘要： Norepinephrine (NE) concentrations in the accessory olfactory bulbs (AOB) of young (4-5 months)and old (25-26 months) ovariectomized Sprague-Dawley rats, which were implanted with a 17 β-estradiolsilastic capsule and then exposed to male rat urine, were studied. The unilateral vomeronasal organ wasremoved in all rats one week before exposure to the urine stimulation. The NE level in the AOB of thissurgical side served as the control. Urine collected from young adult male rats was poured into the female'scage at 12:00h and the animals were sacrificed before and 1, 2, or 3 hours after the male urine was given.The NE basal levels in the AOB of young rats decreased significantly at 14:00h compared to those at12:00h, while no obvious changes in the NE concentrations were observed in the AOB of old rats duringthe same period. Two hours after continuous exposure to male urine, the NE concentrations in the AOBof young rats markedly increased, but no similar response occurred in the old rats. These data suggest thatthe noradrenergic functions in the AOB under basal conditions as well as in response to pheromonal sti-mulation may be modified with increasing age.
• 中文關鍵字： agint; pheromone; accessory olfactory bulb; norepinephrine.
• 英文關鍵字： --