- 作者: Wei Sun; Wen-Wen Shen; Shuang Yang; Fen Hu; Yang Gao; Yu-Huan Qiao; Tian-Hui Zhu
- 作者服務機構: Laboratory of Molecular Genetics, College of Medicine, Nankai University, Tianjin, China
- 中文摘要: --
- 英文摘要:
Background: The human lmo2 gene plays important roles in hematopoiesis and is associated with acute T
lymphocyte leukemia. The gene encodes two protein isoforms, a longer form LMO2-L and a shorter form LMO2-S. Both
isoforms function as bridge molecules to assemble their partners together to regulate their target genes. A typical
LMO2 binding site consists of two elements, a GATA site and an E-box, with an interval of 9~12 bp.
Methods: In this study, the combination of MBP pulldown assay and mammalian two hybrid assay were used to
confirm the homo-binding character of LMO2-L/-S isoforms. Luciferase reporter assay and Real-time PCR assay were
used to detect expression levels and relative promoter activities of LMO2-L/-S isoforms. Co-transfection and Luciferase
reporter assay were used to reveal the detailed regulatory pattern of LMO2-L/-S isoforms on their targets.
Results: Herein we report the homo-interaction character of LMO2-L and LMO2-S and their major difference in
manner of regulating their target genes. Our results showed that LMO2-L and LMO2-S could only bind to themselves
but not each other. It was also demonstrated that LMO2-L could either positively or negatively regulate the
transcription of its different target genes, depending on the arrangement and strand location of the two elements
GATA site and E-box, LMO2-S, however, performed constitutively transcriptional inhibiting function on all target genes.
Conclusion: These results suggest that LMO2 isoforms have independent functions while there is no interaction
between each other and they could play synergetic or antagonistic roles precisely in regulating their different genes
involved in normal and aberrant hematopoiesis. - 中文關鍵字: --
- 英文關鍵字: --