- 作者: 黃思誠; 謝長堯; 歐陽培銓; 陳浩佳
- 作者服務機構: 國立臺灣大學醫學院婦產科; 美國國立衛生院內分秘生殖研究部
- 中文摘要: 本研究證明分析hCG的層析聚等電點法,可以很簡單的建立起來,並且此法對於發現人體體液內hCG的desial-ylation的程度很有效。 為了分析體液內的hcG,層析聚等電點法必須分成pH 6.2~3.0及PH 9.0~6.0兩個階段來做。在層析聚等電點法時,高度純化的hCG(CR121)是酸性的,其等電點為4.22~3.8,而hCGβ印則更酸性,其等電點為4.0~3.2另外第一期妊娠,葡萄胎及絨毛膜癌的_hCG_,主要也是酸性的,因此pH6.2~3.0的層析聚等電點法適合於分析高度純化的_hCG_,hCGβ,以及第一期妊娠,葡萄胎及絨毛膜癌的hCG。另一方面,第三期妊娠,及妊娠毒血症的hCG是鹼性的,因此pH9.0~6.0的層析聚等電點法適合於分析第三期妊娠及妊娠毒血症之hCG。 本研究顯示由層析聚等電點法可知desialylated hCG的部分隨著妊娠的進展而增加,此表示在老化的胎盤,hCG的醣化步驟,尤其sialylation的步驟有障礙。本研究發現葡萄胎及絨毛膜癌的hCG是酸性的,但是文獻上有絨毛膜癌的hCG是desialylated的報告,故絨毛膜癌的hCG值得做更進一步的研究。
- 英文摘要: This study demonstrates that chromatofocusing is powerful in analyzing multiple forms of hCGfrom biological fluids. For analyzing hCG from biological fluids, it is necessary to perform chromatofocus-ing, in the range of pH 6.2-3.0 and pH 9.0-6.0. By chromatofocusing, highly purified hCG (CR121) wasfound to be acidic, in the range of pI 4.22-3.8, and hCGβ was more acidic, in the range of pI 4.0-3.2.Moreover, hCG from the first trimester pregnancy, hydatidiform mole or choriocarcinoma was also mainlyacidic. Therefore, chromatofocusing in the range of 6.2-3.0 was suitable for analyzing purified hCG, hCGβ,and urinary hCG from the first trimester pregnancy, hydatidiform mole and choriocarcinoma. On theother hand, because hCG in the third trimester pregnancy and the toxemia of pregnancy were mainlyalkaline, the chromatofocusing system in the range of pH 9.0-6.0 was suitable for analyzing hCG from thethird trimester pregnancy and the toxemia of pregnancy.
- 中文關鍵字: chromatofocusing; hCG; normal pregnancy; toxemia of pregnancy; choriocarcinoma
- 英文關鍵字: --