- 作者: Tzong-Shiann Ho; Chia-Ying Tsai; Nina Tsao; Nan-Haw Chow; Huan-Yao Lei
- 作者服務機構: a Departments of Microbiology and Immunology, and; b Department of Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
- 中文摘要: --
- 英文摘要: We previously reported that murine experimental allergic encephalomyelitis can be induced by an additional intraperitoneal and intracerebral (i.c.) resti-mulation in resistant B6 mice after standard immunization with myelin antigens in complete Freund's adjuvant and Bordetella pertussis coadjuvant. Neutrophils infiltrated into perivascular spaces at 12 h, followed by mononuclear cells 24 h after i.c. injection. In this study, we report that the i.c. injection induced the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). The kinetic expression of ICAM-1 or VCAM-1 on brain endothelial cells paralleled the infiltration of neutrophils and mononuclear cells, respectively. The infiltrated lymphocytes also expressed very late antigen-4 (VLA-4) molecules. The microvascular endothelial cells were positive for VCAM-1, whereas the surrounding mononuclear cells were VLA-4 positive. Furthermore, we found a unique subpopu-lation of cells with characteristics of CD4-CD8-Vβ8+ markers. The kinetic studies of this population showed that these cells were transiently depleted from 12 to 24 h after i.c. challenge (before the development of clinical symptoms) in cervical lymph nodes. These CD4-CD8-Vβ8+ cells can be expanded by in vitro culture with myelin basic protein or IL-2. No significant changes of CD4+/CD8+ cells were noted. CD4+CD8-CD3 + cells were also found in brain by double histochemical stains and were the major infiltrating cells at 24 or 48 h after i.c. challenge.
- 中文關鍵字: --
- 英文關鍵字: Experimental allergic encephalomyelitis ; Adhesion molecule ; CD4-CD8-CD3+ cells ; Intracerebral stimulation ; Cervical lymphatics