- 作者: 魏琴,李燕,段彩虹,馬洪敏,杜斌
- 作者服務機構: School of Chemistry & Chemical Engineering, Jinan University, Jinan 250022, P. R. China
- 中文摘要: In this paper, the spectral behavior of protein and Poniacyl Carmine 2B (PC 2B) has been studied by spectrophotometric method. The conditional constants, apparent combination constant K and maximum binding number n, were used to express the combination ability of the reactions between PC 2B and protein under a set of given conditions. The Sandell index s was used to express the sensitivity of the determination of protein. The factors, acidity, PC 2B concentration and the ionic strength, were discussed by the change of apparent combination constant and maximum binding number. It was found that acidity of the solution, PC 2B concentration and ionic strength had a significant effect on the sensitivity of the assay of protein. Under the optimal conditions, the apparent combination constant K and the maximum binding number n were 2.36 × 106 L mol-1 and 95, respectively. With further investigation, it was found that the Scatchard model was suitable in treating the data obtained in the experiments. In the buffer medium of HCl-KCl at 1.87, the addition of protein made the maximum absorption of the system move from 527 nm to 513 nm. Its apparent molar absorptivity is 4.46 × 105 L mol-1 cm-1 at 513 nm. Beer’s law is obeyed in the range of 0 ~ 55 μg mL-1. The system developed in this paper has been used for the determination of protein in milk powder successfully.
- 英文摘要: --
- 中文關鍵字: Poniacyl Carmine 2B; Protein; Spectral probe; Binding number; Spectral behavior.
- 英文關鍵字: --