- 作者: Shaw-Wei D Tsen; Chao-Yi Wu; Avedis Meneshian; Sara I Pai; Chien-Fu Hung; T.-c. Wu
- 作者服務機構: Department of Pathology, The Johns Hopkins School of Medicine, Baltimore, MD, USA
- 中文摘要: --
- 英文摘要:
Background:
Gene therapy with plasmid DNA is emerging as a promising strategy for the treatment of many
diseases. One of the major obstacles to such therapy is the poor transfection efficiency of DNA
in vivo.
Methods
In this report, we employed a very low power, near-infrared femtosecond laser technique to
enhance the transfection efficiency of intradermally and intratumorally administered DNA
plasmid.
Results:
We found that femtosecond laser treatment can significantly enhance the delivery of DNA into
the skin and into established tumors in mice. In addition, we found that both laser power density
as well as duration of laser treatment are critical parameters for augmenting DNA transfection
efficiency. The femtosecond laser technique employs a relatively unfocused laser beam that
maximizes the transfected area, minimizes damage to tissue and simplifies its implementation.
Conclusions:
This femtosecond new laser technology represents a safe and innovative technology for
enhancing DNA gene transfer in vivo. - 中文關鍵字: --
- 英文關鍵字: --