• 作者： 丁予安; 張維國; 陳懋良
• 作者服務機構： 三軍總醫院內科學科心臟科; 國防醫學院公共衛生學科; 國防醫學院生化學科
• 中文摘要： 本實驗係改變大白鼠飲食中脂肪酸之成份，來探討其對血清及血小板細胞膜脂肪酸的影響，進而研究其對血小板之凝集作用。將五十五隻大白鼠分為五組，分別為：控制組、豬油組、豬油加魚油組、黃豆油組，及黃豆油加魚油組，以膽固醇為l％及上述各種脂肪酸來餵食，進行五十六天的實驗。凡餵食魚油之各組，其血清中arachidonic acid（20:4, w-6,AA）減少，eicosapentaenoio acid（20：5, w-3, EPA）增加；在餵食多元不飽和脂肪酸的各組，其血小板細胞膜上之磷脂質亦有相同的變化。血小板凝集反應的測定，係利用血小板凝集反應記錄儀，以不同濃度之ADP誘發凝集反應後，測量凝集曲線的斜率及高度；凡餵食魚油之各組皆有明顯地抑制凝集作用，此種抑制作用可能與飲食增加了血小板細胞膜上磷脂質中的EPA與AA之比例有關。血小板凝集作用與凝集素（ThromboxanoB ）濃度，在豬油及豬油加魚油組的變化要較黃豆油及黃豆油加魚油組為明顯。而　malondialdehyde（MDA）的濃度在五組之間皆無明顯的差異存在。
• 英文摘要： In the present study, we changed the fatty acid profile in blood and platelet membranes by dietarymanipulation, and examined the effect on platelet aggregation in rats. Fifty-five rats were divided intofive groups and fed for 56 days with 1% cholesterol and different types of fatty acid-rich diets: basal,lard, lard + fish oil, soybean oil, and soybean oil＋fish oil. A decrease in serum arachidonic acid (20:4,ω-6, AA) and an increase in serum eicosapentaenoic acid (20:5, ω-3, EPA) were found in all experimentaldietary groups fed with refined fish oil. Similar changes in the polyunsaturated fatty acids were alsofound in the platelet membrane phospholipids. Platelet aggregation, quantitated by the slope and heightof the aggregation curve induced by different concentrations of ADP in a platelet aggregometer, was in-hibited in all groups fed with refined fish oil. This inhibition of platelet aggregation may be related to anincrease in the ratio of EPA and AA in the platelet membrane phospholipids after dietary manipulation.The differences in the platelet aggregation and thromboxane B (TXB ）concentration between the lardand the lard + fish oil groups were more profound than that between the soybean oil and the soybean oil+ fish oil group. However, the malondialdehyde (MDA) concentration revealed no significant differencesbetween the five groups.
• 中文關鍵字： refined fish oil; polyunsatruated fatty acid; platelet aggregation; thromboxane
• 英文關鍵字： --