- 作者: Ching-Hsiung Lin; Kun-Tu Yeh; Ya-Sian Chang; Nicholas C Hsu; Jan-Gowth Chang
- 作者服務機構: Department of Chest Medicine, Changhua Christian Hospital, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Epidermal growth factor receptor (EGFR) kinase domain mutations hyperactivate the
kinase and confer kinase addiction of the non-small-cell lung cancer (NSCLC) tumor cells.
Almost all of these mutations are located within exons 18-21. The −216 single nucleotide
polymorphism in the promoter region is associated with increased EGFR production. We
present a method for detecting these common mutations in 81 cases of NSCLC. The
protocol is based on the multiplex amplification of promoter region and exons 18 −21 of
the EGFR genes in a single tube, followed by primer extension of the PCR products using
various sizes of primers to detect base changes at −216 promoter region and codons 719,
746-750, 790, 858 of the EGFR gene. We compared the results with that from direct
sequencing for detecting EGFR mutations in 81 cases of NSCLC. The two methods
identified the same 26 mutations, but our method is superior to direct sequencing in terms
of the amount of work and time required. We presented a simple and fast method to detect
mutations of EGFR genes in NSCLC. - 中文關鍵字: --
- 英文關鍵字: --