- 作者: Mohamed H. Abdel-Hay, Ekram M. Hassan, Azza A. Gazy and Tarek S. Belal*
- 作者服務機構: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, University of Alexandria, Elmessalah 21521, Alexandria, Egypt
- 中文摘要:
Simple, reliable, sensitive and accurate kinetic spectrophotometric and spectrofluorimetric methods
were proposed for the determination of ciprofloxacin hydrochloride (CPX) and norfloxacin (NRX) in
pure form and in pharmaceuticals. The methods are based on coupling the studied drugs with 4-chloro-7-
nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in the presence of alkaline borate buffer. Spectrophotometric
measurement was achieved by recording the absorbance at 477 nm after a fixed time of 20 and 15 min on a
water bath adjusted at 70 1 C for CPX and NRX, respectively. The same product exhibited emission
peaks at 540 nm. The different experimental parameters affecting the development and stability of the
color were carefully studied and optimized. The absorbance concentration plots were linear over the
ranges 3-18 and 2.5-15.0 g/mL for CPX and NRX, respectively, while the fluorescence concentration
plots were linear over the ranges 0.06-0.36 and 0.05-0.30 g/mL for CPX and NRX, respectively. The
limit of detection of the kinetic method was about 0.2 g/mL for both drugs while the fluorescence measurement
enabled their detection at a concentration of about 0.012 g/mL. The proposed methods were
successfully applied for the assay of the two drugs in their commercial products. The results obtained were
statistically compared with those obtained by reference HPLC and spectrophotometric methods. The
stoichiometry of the reaction was determined and the reaction pathway was postulated. - 英文摘要: --
- 中文關鍵字: Ciprofloxacin hydrochloride; Norfloxacin; NBD-Cl; Kinetic spectrophotometry; Spectrofluorimetry; Pharmaceutical preparations.
- 英文關鍵字: --