- 作者: Lie-Fen Shyur; Chih-Huai Chen; Chiu-Ping Lo; Sheng-Yang Wang; Pei-Ling Kang; Show-Jane Sun; C.ALLEN CHANG; Chi-Meng Tzeng; Ning-Sun Yang
- 作者服務機構: Institute of BioAgricultural Sciences, Academia Sinica, and Department of R&D, U-Vision Biotech Inc., Taipei, and Department of Biological Science and Technology, National Chiao Thun University, Hsinchu, Taiwan, ROC
- 中文摘要: --
- 英文摘要: The antitumor activity of Anoectochilus formosanusHayata(Orchidaceae), a popularly used folk medicine inthe treatment of cancers in Asia,was investigated inMCF-7 human mammary carcinoma cells. Plant extractsof A. formosanus were observed to induce apoptosisof MCF-7 cells as evidenced by cell-morphologicalchanges, an early redistribution of plasma membranephosphatidylserine, and DNA content distribution stud-ies. Bioactivity-guided fractionation of A. formosanusextracts produced a specific ethyl acetate(EA)-parti-tioned fraction in which apoptotic activity was enriched.The chemical profile and candidate index compounds ofthe active EA fraction were obtained using HPLC and var-ious spectral analyses.Western blot analysis showedthat upon treatment of MCF-7 cells with the EA fraction,cleavage of pro-caspases-8, -9, and -7, and poly(ADP-ribose)polymerase as well as significant release of mito-chondrial cytochrome c into the cytosol were readilyobserved. Flow cytometry showed that the Fas ligandprotein was overexpressed in EA-treated MCF-7 cells.Functional genomic studies indicated that specific genesrelated to cytoskeleton rearrangement, apoptotic signaltransduction,and various transcription factors were dif-ferentially regulated in EA-treated MCF-7 cells. Putativeapoptotic signaling pathways of MCF-7 cells in responseto the EA extract of A. formosanus are proposed.
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- 英文關鍵字: --