- 作者: 郭光雄; 陳秀男; 鍾虎雲
- 作者服務機構: 國立臺灣大學動物研究所
- 中文摘要: 將患有凹凸病之鰻魚體上病巢附近的肌肉組織,在高速下打碎,經density gradientcentrifugation方法分離出微孢子蟲Plistophora anguillarum,混合adjuvant後經皮下注射免疫兔體,產生凝集價高之血清,經r-Globulin之分層(fractionation)與FITC標識後得到效價20之螢光抗體(Conjugate),此螢光抗體以直接螢光抗體法檢查患凹凸病之幼鰻,能成功地將肌肉中存在之孢子或孢子囊體(Cyst)染上螢光,且具有特異性(Specificity)。
- 英文摘要: Plistophora anguillarum is a microsporidian parasite inhabiting in themuscular tissues of the eel,Anguilla japonica. The muscle of infected areawas homogenized and a P. anguillarum preparation was purified with densitygradient centrifugation. The preparation immersified with the adjuvant wasimmunized to healthy rabbits with subcutaneous injections. The r-Globulinwas fractionated from the rabbit serum of high agglutination titers, andwas labelled with FITC. The titer(1:20) was obtained by direct fluorescentantibody test, and it could be used successfully and specifically for thedetection of spores or cysts in the infected muscles of young eel.
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