• 作者： 王志祿; 蘇慕寰; 趙祖怡; 蕭孟芳; 楊崑德
• 作者服務機構： 三軍總醫院小兒部內科部; 國防醫學院藥學系; 國防醫學院熱帶及寄生蟲學系
• 中文摘要： 老鼠及囓鼠類之巨噬細胞釋放一氧化氮被認為與腫瘤毒殺及細胞內微生物之清除關係密切。目前證據顯示人類單
  核球細胞所衍生之巨噬細胞或骨髓性血癌單核球細胞株並不會持續釋放一氧化氮。我們之實驗結果顯示，人類單核球細
  胞所衍生之巨噬細胞經脂肪多醣類(lipopolysaccharide)刺激之後，可產生一氧化氮，但比老鼠巨噬細胞所產生之量為少
  (1.26 ± 0.20 vs 42.5 ± 6.3 μM)。在不同之體外及體內狀況下，人類巨噬細胞釋放一氧化氮之程度有所不同。在塑膠培
  養皿中培養七天後之人類單核球衍生巨噬細胞比在鐵弗龍(Teflon)培養皿中釋放較多量之一氧化氮(1.22 ± 0.13 vs 0.14
  ± 0.07 μM)。除此之外，由不同疾病狀況下取得之人類居住性巨噬細胞產生一氧化氮具有鈣離子及蛋白質激?依賴之
  特性。這與老鼠巨噬細胞產生一氧化氮具有鈣離子非依賴性及磷酸?依賴之特性有所不同。由以上之實驗結果顯示，
  人類巨噬細胞釋放一氧化氮之機轉不僅與老鼠並不相同，且在各種不同的體外及體內狀況下產生一氧化氮之程度亦有所
  差異。
• 英文摘要： Nitric oxide (NO) release from mouse and rat macrophages is implicated in tumor cell cytotoxicityand the killing of intracellular organisms. Evidence, however, suggests that human monocyte-derivedmacrophages or myeloid leukemic cells differentiated along the monocytic lineage do not consistentlyrelease NO. Results presented herein that human monocyte-derived macrophages in response to lipopolysac-charide can release NO, but markedly less than mouse macrophages (1.26 ± 0.20 vs 42.5 ± 6.3 M). Differentin vitro and in vivo conditions have variable effects on NO production by human macrophages. Mono-cytes cultured in plastic wells, but not teflon beakers, for 7 days can release NO (1.22 ± 0.13 vs 0.14 ± 0.07 M).In addition, human resident macrophages derived from patients with certain diseases possess a calcium- andprotein kinase C-dependent pathway to produce NO, in contrast to the calcium-independent and phos-phatase-dependent NO release in murine macrophages. Above results suggest that the mechanism ofproducing NO by macrophages in humans is not only different from that in mice, but also depends oncertain in vitro and in vivo conditions.
• 中文關鍵字： macrophage; nitric oxide.
• 英文關鍵字： --