第7卷‧第4期,
198310
, pp. 243-248
放射免疫測定法直接測定血中癌胚胎抗原
- 作者:
黃敏和; 周成功; 趙遐父
- 作者服務機構:
榮民總醫院胃腸科; 榮民總醫院醫學研究部
- 中文摘要:
本實驗室由腸癌轉移至肝臟的組織中,純化出癌胚胎抗原,經由SDS膠電泳及免疫電泳看出此抗原為單一的蛋白質。利用此抗原製造抗體,並發展成放射免疫測定法,直接測定病人血液中癌胚胎抗原,以雙重抗體法沈殿結合的癌胚胎抗原。本放射免疫測定法的靈敏度為1.76 ng/ml。四十個病人的血清由本法及由Sorin買來的癌胚胎抗原測定組分別測定,以直線回歸比較兩法測定結果,發現有很好的相關性。本法直接測定血清,不需要預光處理,操作過程非常簡單,而且可靠性也很好。
- 英文摘要:
Carcinoembryonic antigen was purified from liver metastasis of colonic adenocarcinoma in this labora-tory. This molecule is shown homogeneous on SDS slab gel electrophoresis and immunoelectrophoresisreacted with the antiserum raised by the same antigen. A direct radioimmunoassay of this antigen withcompetitive inhibition system was established. The free and bound CEA were separated by use of a secondantibody. The present radioimmunoassay has the sensitivity of 1.76 ng/ml and shows excellent correlationwith the commercial CEA kit from Sorin. This direct radioimmunoassay for carcinoembryonic antigenis very simple and reliable.
- 中文關鍵字:
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- 英文關鍵字:
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