- 作者: P. Jia; C. Zhang; X. P. Huang; M. Poda; F. Akbas S. L. Lemanski; N. Erginel-Unaltuna; L. F. Lemanski
- 作者服務機構: Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
- 中文摘要: --
- 英文摘要:
The discovery of the naturally occurring cardiac non-function (c) animal strain in Ambystoma mexicanum (axolotl) provides a valuable animal model to study cardiomyocyte differentiation. In homozygous mutant animals (c/c), rhythmic contractions of the embryonic
heart are absent due to a lack of organized myofibrils. We have previously cloned a partial sequence of a peptide cDNA (N1) from an anterior-endoderm-conditioned-medium RNA library that had been shown to be able to rescue the mutant phenotype. In the current studies we have fully cloned the N1 full length cDNA sequence from the library. N1 protein has been detected in both adult heart and skeletal muscle but not in any other adult tissues. GFPtagged expression of the N1 protein has revealed localization of the N1 protein in the endoplasmic reticulum (ER). Results from in situ hybridization experiments have confirmed the dramatic decrease of expression of N1 mRNA in mutant (c/c) embryos indicating that the N1 gene is involved in heart development. - 中文關鍵字: --
- 英文關鍵字: Ambystoma mexicanum; N1 gene; In situ hybridization; ER retention signal; Myofibrils