- 作者: Annalisa Palmieri; Furio Pezzetti; Giorgio Brunelli; Ilaria Zollino; Luca Scapoli; Marcella Martinelli; Marzia Arlotti; Francesco Carinci
- 作者服務機構: 1 Institute of Histology, University of Bologna, Bologna, Italy; ; 2 Center of Molecular Genetics, CARISBO Foundation, University of Bologna, Bologna, Italy; ; 3 Department of DMCCC, Section of Maxillofacial Surgery, University of Ferrara, Corso Giovecca, 203, Ferrara, 44100, Italy
- 中文摘要: --
- 英文摘要: PerioGlas (PG) is an silicate-based (i.e. anorganic) material used for grafting periodontal osseous defects since the ninety whereas P-15 is an analog of the cell binding domain of collagen (i.e. organic material) that is successfully used in clinical trial to promote bone formation. However, how PG (i.e anorganic material) and P-15 (i.e. collagen) differentially alter osteoblast activity to promote bone formation is unknown. We therefore attempted to get more insight by using microRNA microarray techniques to investigate the translation process in osteoblasts differentially exposed to PG and P-15. We identified 3 up-regulated miRNA (i.e. mir-30b, mir-26a, mir-92) and 8 down-regulated miRNA (i.e. mir-337, mir-377, mir-25, mir-200b, mir-129, mir-373, mir-133b, mir-489). The data reported are, to our knowledge, the first study on translation regulation in osteoblatsts differentially exposed to cell binding domain of collagen and to silicate-based material. Both enhance the translation of several miRNA belonging to osteogenetic genes, but P-15 acts preferentially on homeobox genes.
- 中文關鍵字: --
- 英文關鍵字: alloplastic material, allograft, miRNA, microarray, gene expression, gene profiling