- 作者: 朱達德 ; 李丁在 ; 魏國晉 等
- 作者服務機構: 台灣大學化學系
- 中文摘要: Glycoconjugates, such as chromophore-labeled disaccharides and permethylated glycosphingolipids (GSL) were used for comparison of triple quadrupole and double focusing mass spectrometers in analysis of product ions. A profound effect of collision energy was observed in the product ion spectra of ceramide ions (fragment ions of permethylated GSL): more product ions were observed from a double focusing mass spectrometer. Besides collision energy, the structure of the analyte had a significant effect on the formation of product ions. Despite the fact that masses of protonated molecular ions (MH/sup +/) of permethylated GSL are significantly larger than their ceramide fragments, the low-energy and high-energy product ion spectra of MH/sup + / are similar generally. In a double focusing mass spectrometer of reversed geometry, more metastable ions were observed in the first field free region (FFR) than in the second FFR. The metastable ions observed in the second FFR were similar to those observed in low-energy collision- induced dissociation (CID). Although a double focusing mass spectrometer is superior to triple quadrupole instrument for detection of product ions, the poor resolution in either the selection of precursor ion or in the product ion spectra can be a serious problem in analysis of a mixture with similar masses.
- 英文摘要: --
- 中文關鍵字: Triple Quadrupole; Double Focusing Mass Spectrometer; Collision-Induced Dissociation (Cid); Metastable Ion; Mass Analyzed Ion Kinetic Energy Spectroscopy (Mikes)
- 英文關鍵字: 二次聚焦質譜測定術;碰撞誘發解離;半穩定離子;質量分析離子動能光譜分析