- 作者: Yi Chang; Ta-Liang Chen; Gong-Jhe Wu; George Hsiao; Ming-Yi Shen; Kuan-Hung Lin; Duen-Suey Chou; Chien-Huang Lin; Joen-Rong Sheu
- 作者服務機構: Department of Anesthesriology, Shin Kong Wu Ho-Su Memorial Hospital, and Departments of Anesthesiology and Pharmacology, and Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: The aim of this study was to systematically examine theinhibitory mechanisms of ketamine in platelet aggrega-tion. In this study, ketamine concentration-dependently(100-350 M) inhibited platelet aggregation both inwashed human platelet suspensions and platelet-richplasma stimulated by agonists. Ketamine inhibited phos-phoinositide breakdown and intracellular Ca2+ mobiliza-tion in human platelets stimulated by collagen. Ketamine(200 and 350 M) significantly inhibited thromboxane(Tx) A2 formation stimulated by collagen. Moreover,ke-tamine (200 and 350 M)increased the fluorescence ofplatelet membranes tagged with diphenylhexatriene.Rapid phosphorylation of a platelet protein of Mr47,000(P47), a marker of protein kinase C activation, wastriggered by phorbol-12,13-dibutyrate(100 nM). Thisphosphorylation was markedly inhibited by ketamine(350 M). These results indicate that the antiplateletactivity of ketamine may be involved in the followingpathways. Ketamine may change platelet membranefluidity, with a resultant influence on activation of phos-pholipase C, and subsequent inhibition of phosphoinosi-tide breakdown and phosphorylation of P47, therebyleading to inhibition of intracellular Ca2+ mobilizationand TxA2 formation, ultimately resulting in inhibition ofplatelet aggregation.
- 中文關鍵字: --
- 英文關鍵字: --