- 作者: Junkun He; Leonard N. Binn; Sergei A. Tsarev; Curtis G. Hayes; John A. Frean; Margaretha Isaacson; Bruce L. Innis
- 作者服務機構: Department of Virus Diseases, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, and Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Md., USA; South Africa Institute for Medical Research and University of the Witwatersrand, Johannesburg, South Africa
- 中文摘要: --
- 英文摘要: Hepatitis E virus (HEV) causes sporadic and epidemicacute viral hepatitis in many developing countries. InAfrica, hepatitis E has been documented by virus detec-tion (reverse transcriptase polymerase chain reaction,RT-PCR) in Egypt, Chad, Algeria, Morocco and Tunisia.Cases of presumptive hepatitis E also have been docu-mented by detection of antibody to HEV in the Sudan,Kenya, Ethiopia, Somalia, Djibouti and South Africa. Re-cently, we reported the recovery of 9 isolates of HEVfrom feces collected during an outbreak of jaundice inNamibia. These specimens were stored frozen for manyyears at the South African Institute for Medical Researchawaiting new methods to determine the etiology of jaun-dice. HEV genomic sequences were detected by antigen-capture RT-PCR with primers that amplified 2 indepen-dent regions of the HEV genome (ORF-2 and ORF-3). Tofurther characterize the HEV 83-Namibia isolates, wedetermined the nucleotide (nt) sequence of the 3' end ofthe capsid gene (296 of 1,980 nt in ORF-2) and ORF-3 for1 isolate. The capsid gene sequence shared 86% identitywith the prototype Burma strain and up to 96% identitywith other African strains at the (nt) level, and 99% identi-ty with Burma or other Africa strains at the amino acidlevel. A 188 (nt) fragment amplified from ORF-3 was alsohighly homologous to other HEV but was too short formeaningful comparison. Phylogenetic analysis indicatedthat HEV 83-Namibia is closely related to other Africanisolates, and differs from Burmese, Mexican and ChineseHEV. These data link the HEV causing the 1983 Namibiaoutbreak to more recent HEV transmission in northernand sub-Saharan Africa, suggesting this subgenotype ofHEV is firmly established throughout the continent.
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