- 作者: Ying-Ying Lu, Tong-Sheng Chen, Jun-Le Qu, Wen-Liang Pan, Lei Sun, Xun-Bin Wei
- 作者服務機構: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou, China
- 中文摘要: --
- 英文摘要:
Background:
Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the
traditional Chinese herb Artemisia annua, is recommended as the first-line anti-malarial
drug with low toxicity. DHA has been shown to possess promising anticancer activities
and induce cancer cell death through apoptotic pathways, although the molecular
mechanisms are not well understood
Methods: In this study, cell counting kit (CCK-8) assay was employed to evaluate the survival of
DHA-treated ASTC-a-1 cells. The induction of apoptosis was detected by Hoechst
33258 and PI staining as well as flow cytometry analysis. Collapse of mitochondrial
transmembrane potential (m) was measured by dynamic detection under a laser
scanning confocal microscope and flow cytometry analysis using Rhodamine123.
Caspase-3 activities measured with or without Z-VAD-fmk (a broad spectrum caspase
inhibitor) pretreatment by FRET techniques, caspase-3 activity measurement, and
western blotting analysis.
Results:
Our results indicated that DHA induced apoptotic cell death in a dose- and
time-dependent manner, which was accompanied by mitochondrial morphology
changes the loss of m and the activation of caspase-3.
Conclusions:
These results show for the first time that DHA can inhibit proliferation and induce
apoptosis via caspase-3-dependent mitochondrial death pathway in ASTC-a-1 cells. Our
work may provide evidence for further studies of DHA as a possible anticancer drug in
the clinical treatment of lung adenocarcinoma. - 中文關鍵字: --
- 英文關鍵字: --