- 作者: Lawrence S. Chin; S atyendra K. Singh; Qiang Wang; Susan F. Murray
- 作者服務機構: Departiment of Neurosurgery and Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimor, Md., USA
- 中文摘要: --
- 英文摘要: To identify novel genes associated with apoptosis inglioma cells, we treated T98G glioma cells with okadaicacid (OA). Differential display using 15 random primerswas performed on RNA extracted from these cells.Upregulated bands were excised from polyacrylamidegels and cloned. Northern blots were used to confirmRNA expression in T98G cells. 18 RNA fragments corre-sponding to the untranslated region of genes were iden-tified and sequenced. Three unknown gene fragmentswere used to screen a fetal brain cDNA library resultingin three complete cDNA sequences. The three sequencescorresponded to a human gene homologous to the yeasttranslation initiation factor Sui-1, a cAMP-regulatedphosphoprotein, ARPP-16/19, and a novel gene designat-ed 048. Transcription of Sui-1 increased in response toall stress factors tested, whereas ARPP only respondedto OA. 2-kb and 4-kb 048 RNA species were identified.OA and stress factors increased 2-kb expression whileK252a (protein kinase inhibitor) increased 4-kb expres-sion. Differential display is effective for identifying genesassociated with apoptosis. Novel genes may be identi-fied by further analysis of the gene fragments identifiedin this study. The function of 048 is unknown.
- 中文關鍵字: --
- 英文關鍵字: --