- 作者: Savitha Balakrishnan; Peeyush KUMAR T; Paulose C S
- 作者服務機構: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, INDIA.
- 中文摘要: --
- 英文摘要:
Metabotrophic glutamate receptors (mGluRs) modulate cellular activities
involved in the processes of differentiation and degeneration. In this study, we have
analysed the expression pattern of group-I metabotropic glutamate receptor (mGlu-5) in
cerebral cortex, corpus striatum, brainstem and hippocampus of streptozotocin induced
and insulin treated diabetic rats (D+I) as a function of age. Also, the functional role of
glutamate receptors in intra cellular calcium release from the pancreatic islets was studied
in vitro. The gene expression studies showed that mGlu-5 mRNA in the cerebral cortex
increased siginficantly in 7weeks old diabetic rats whereas decreased expression was
observed in brainstem, corpus striatum and hippocampus when compared to control.
90weeks old diabetic rats showed decreased expression in cerebral cortex, corpus
striatum and hippocampus whereas in brainstem the expression increased significantly
compared to their respective controls. In 7weeks old D+I group, mGlu-5 mRNA
expression was significantly decreased in cerebral cortex and corpus striatum whereas the
expression increased significantly in brainstem and hippocampus. 90weeks old D+I
group showed an increased expression in cerebral cortex, while it was decreased
significantly in corpus striatum, brainstem and hippocampus compared to their respective
controls. In vitro studies showed that glutamate at lower concentration (10-7M) stimulated
calcium release from the pancreatic islets. Our results suggest that mGlu-5 receptors have
differential expression in brain regions of diabetes and D+I groups as a function of age.
This will have clinical significance in management of degeneration in brain function and
memory enhancement through glutamate receptors. Also, the regulatory role of glutamate
receptors in calcium release has immense therapeutic application in insulin secretion and
function. - 中文關鍵字: --
- 英文關鍵字: --